Protection Effect and Mechanism of Huangshan Maofeng Tea Polyphenols Extracts on CCl4-Induced Liver Injury

Abstract

The purpose of this study is to observe whether Huangshan maofeng tea polyphenols extracts have protection effect for mice liver injuries caused by carbon tetrachloride (CCl4) and its mechanism. Method: High (300 mg/kg), middle (150 mg/kg) and low (75 mg/kg) doses of Huangshan maofeng tea polyphenols were administered for 7 days. One hour after the last gavage, all groups (except the control group) will inject in peritoneal 0.10% CCl4 olive oil solution. After 24 hours, blood from eyeball and liver tissue will be extracted. Changes in content of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) will be detected; The expression of the mRNAs and proteins of the four liver enzymes were detected using Real-Time PCR and Western Blot. Result: Huangshan maofeng tea polyphenols low, medium, high dose groups can all decrease the activity of ALT and AST in serum of mice with acute liver injury; Huangshan maofeng tea polyphenols low, medium, high dose groups can all induce mice liver Cyp3a11 mRNA and protein expression. Conclusion: Huangshan maofeng tea polyphenols extracts can protect liver by inducing the expression of Cyp3a11.